Abstract
1. A method is described that enables one to collect with a micropipette cells from several hundred early medaka ( Oryzias latipes) embryos in less than 30 min; the cells can subsequently be dissociated by ethylenediamine tetraacetic acid. 2. d-Tubocurarine (10 μM) decreased and carbamylcholine (10 μM) increased the rate of aggregation of these cells in vitro. 3. d-Tubocurarine also decreased the proportion of cells that translocated a measurable distance in vitro. 4. Extracts of the cells contain acetylcholine, and the amount they contain is about 25% of that present in whole embryos. 5. No acetylcholine was detected in the culture medium bathing aggregating cells.
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