Abstract
Whole-cell voltage-clamp recordings from dissociated hair cells of the statocyst of octopus, Eledone cirrhosa, demonstrated that application of ACh, carbachol or muscarine (10 μM) reversibly decreased the amplitude of L-type calcium current (I Ca,L), while nicotine (10–100 μM) did not have any effect. Furthermore, atropine blocked the effect of ACh and agonists suggesting that ACh reduces I Ca,L through activation of muscarinic receptors. Internal dialysis of these cells with guanosine 5′- O-3-thiotriphosphate (GTPγS), a non-hydrolysable GTP analogue, mimicked the ACh-induced inhibition of I Ca,L and occluded any further ACh-induced inhibition. Internal dialysis of these hair cells with guanosine 5′- O-(2-thiodiphosphate) (GDPβS) reduced the ACh-induced inhibition I Ca,L. The inhibitory effects of ACh were abolished by pre-incubation of these cells with pretussis toxin (PTX) suggesting that ACh-induced inhibition of I Ca,L involves a PTX-sensitive G protein pathway.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
