Abstract
Abstract : Using neuroblastoma cells in tissue culture, the transport and metabolism of the acetylcholine (ACh) precursor, choline was examined. Acetylcholinesterase was inhibited so that any ACh formed would not be hydrolyzed enzymatically. Although the transport constant (KT) and capacity (Vmax) are similar to those previously reported, the disposition of choline which is taken up is not that expected for neural cells. Very little (3H)-acetylcholine or free (3H) choline is found in the cells after brief exposure to choline labeled with 3H. Instead, most of the label is found in phosphoryl-(3H) choline which serves as a precursor for (3H) phosphatidyl choline. On the basis of these data and the effects of inhibitors of transport, it is concluded that clones of neuroblastoma cells do not possess the type of choline transport system unique to cholinergic neural tissues, although they are still useful for the study of acetylcholinesterase synthesis. (Modified author abstract)
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