Abstract
It has been established for several years that certain animal species possess an enzyme system capable of oxidizing choline to betaine. The activity of this enzyme in the chick has, at least until recently, been a matter of some dispute. Experiments of Colowick, reported by Jukes and Welch (1942) indicated an increased oxygen uptake by chick liver tissue in the presence of choline or arsenocholine. However, a report by Dubnoff (1949) stated that the young chick does not possess this enzyme. Dinning et al. (1949) reported its occurrence in the mature fowl and more recently Dubnoff (1950) has presented data which were interpreted as indicating an active choline oxidase in chick tissues.In the course of studies dealing with the effect of vitamin B12 on the metabolism of compounds carrying labile methyl groups, we have had occasion to determine the choline oxidase activity of chick liver tissue. Chicks deficient in .
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