Cholestyramine-enhanced fecal elimination of carbon-14 in rats after administration of ammonium [ 14C]perfluorooctanoate or potassium [ 14C]perfluorooctanesulfonate

Abstract

After a single intravenous dose of ammonium [ 14C]perfluorooctanoate ([ 14C]PFO, 13.3 mg/kg) or of potassium [ 14C]perfluorooctanesulfonate ([ 14C]PFOS, 3.4 mg/kg) to rats, cholestyramine fed daily as a 4% mixture in feed was shown to increase the total carbon-14 eliminated via feces and to decrease liver concentration of carbon-14. Rats were fed cholestyramine in feed for 14 days after administration of [ 14C]PFO and for 21 days after administration of [ 14C]PFOS. Control rats were administered radiolabeled fluorochemical but were not treated with cholestyramine. Cholestyramine treatment increased mean cumulative carbon-14 elimination in feces by 9.8-fold for rats administered [ 14C]PFO and by 9.5-fold for rats administered [ 14C]PFOS. After [ 14C]PFO, a mean of 4% of the dose of carbon-14 was in liver of cholestyramine-treated rats at 14 days versus 7.6% in control rats; after [ 14C]PFOS, 11.3% of the dose was in liver at 21 days versus 40.3% in control rats. After administration of either radiolabeled compound, plasma and red blood cell carbon-14 concentrations, which were relatively lower than liver concentrations, were also significantly reduced by cholestyramine treatment.