Abstract
Radiation inactivation was used to determine the functional M r of cholesteryl ester transfer protein (CETP) in rabbit plasma from control and irradiated animals. This technique reveals the size of the functional unit required to carry out the transfer function. The functional M r was calculated to be 70000 ± 3000 (mean ± SD) for both control and irradiated rabbits. This result is in. accordance with the M r obtained by a completely different method, namely SDS-polyacrylamide gel electrophoresis of a partially purified (110-fold) rabbit CETP. The p I of this CETP was found by isoelectric focusing to be equal to 5.95. The results suggest that the functional unit of this enzyme is the monomer.
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