Abstract
Whatever steroids are involved in the pathogenesis of adrenal-regeneration hypertension (ARH), cholesterol side-chain cleavage (SCC) activity is a prerequisite for their synthesis. Both cholesterol SCC and 11β-hydroxylase activities were compared in our experiments. By combining the use of cyanoketone, a 3β-ol dehydrogenase inhibitor, with a sensitive radioimmunoassayfor pregnenolone, we were able to measure pregnenolone formed from endogenous cholesterol in adrenal mitochondrial incubations. The results from a quiescent sacrifice indicated greater SCC activity in the ARH group (0.75 nmoles prognenolone⁄minute⁄mg protein) than in controls (0.45 nmoles pregnenolone⁄minute⁄mg protein). This was related to the significantly greater (p < 0.001) amount of SCC cytochrome P-450 in the high spin state observed in adrenal mitochondria from the ARHgroup (determined by the pregnenolone-induced modified Type II spectral, change). After ether stress, both control and ARH groups had comparableamounts of high-spin SCC c...
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