Abstract
Cholesterol is essential for exocytosis in secretory cells, but distinguishing contributions from lateral organization and dynamics of membrane proteins and stabilization of fusion pores by intrinsic curvature and other mechanical effects of cholesterol have been elusive. The direct effect of cholesterol on fusion pore formation was examined between synaptobrevin 2 (VAMP 2) containing proteoliposomes and an acceptor SNARE complex containing planar supported bilayer using both membrane and content fluorescent markers. This revealed that increasing cholesterol in either the planar supported bilayer or in the synaptobrevin proteoliposome decreases the amount of hemi-fusion and increases the amount of full-fusion with minimal effects on the fusion kinetics.
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