Cholesterol metabolism and sterol carrier protein-2 (non-specific lipid transfer protein)

Abstract

1. Hepatic sterol carrier protein-2 significantly enhances the microsomal conversion of cholesterol to 7α-hydroxy-cholesterol. In the present work we have attempted to correlate the hepatic content of sterol carrier protein-2 with bile acid formation. 2. We have determined the amount of this protein in a variety of physiological and experimental conditions, in which the rate of bile acid synthesis varies over a wide range, viz. (i) during fetal development, (ii) in inbred strains of rats with different rates of bile acid synthesis, and (iii) in rats fed diets containing drugs which modify the rate of bile acid synthesis. 3. The outcome of these experiments does not support the idea that sterol carrier protein-2 has any association with bile acid synthesis. 4. From our data we further conclude that hepatic sterol carrier protein-2 is an adaptable protein because its level increases during development from the fetal to the post-weaning stage of the rat and since it can be modulated by oral administration of certain drugs. 5. Furthermore, it is demonstrated that the level of sterol carrier protein-2 varies between six inbred strains of rats.