Abstract
Mercury ion is one of the most widespread heavy metal contaminants which can accumulate in the body through multiple channels, posing a detrimental impact on human health. We demonstrate a simple and low-cost method for the detection of Hg2+ assisted by a cholesteric liquid crystal photonic hydrogel (polyacrylic acid (PAA)) film with immobilized urease (CLC-PAAurease film). In the absence of Hg2+, a significant change in color and an obvious red shift in the reflected light wavelength of the prepared film were observed, since urease can hydrolyze urea to produce NH3, resulting in an increasing pH value of the microenvironment of CLC-PAAurease film. Hg2+ can inhibit the activity of urease so that the color change of the film is not obvious, corresponding to a relatively small variation of the reflected light wavelength. Therefore, Hg2+ can be quantitatively detected by measuring the displacement of the reflected light wavelength of the film. The detection limit of Hg2+ is about 10 nM. This approach has a good application prospect in the monitoring of heavy metal ions in environmental water resources.
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