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Abstract
Cholera toxin B subunit (CTB) was entrapped in poly(lactide-co-glycolide) microparticles and the responses induced by microencapsulated CTB were comparable to those induced by oral immunization with CTB mixed with whole cholera toxin. In addition, the CTB was released from microparticles intact following in vitro incubation, as determined by the ability of the CTB to bind to its cellular receptor, GM1. Moreover, microencapsulated CTB induced serum antibodies against intact CTB and not against monomeric fragments.
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