Cholera rapid diagnostic tests recycled for PCR confirmation

Amanda K Debes,Wensheng Luo,Ethel Waswa,Waqo Boru,David A Sack

doi:10.1016/s2214-109x(21)00465-4

Amanda K Debes, Wensheng Luo + Show 3 more

Open Access

https://doi.org/10.1016/s2214-109x(21)00465-4

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Abstract

Cholera rapid diagnostic tests recycled for PCR confirmation

Highlights

Detecting and confirming a diagnosis of cholera is challenging for remote areas where culture methods are not readily available
Faecal samples that are spotted on filter paper and dried can be used for PCR confirmation.[1,3]
We explored whether DNA from residual faecal matter on rapid diagnostic tests could be extracted for PCR confirmation

Summary

Introduction

Detecting and confirming a diagnosis of cholera is challenging for remote areas where culture methods are not readily available. Rapid diagnostic tests are available in which a dipstick placed into a diluted stool sample provides results within 15 min;[1,2] because of the performance of rapid diagnostic tests, in terms of specificity, positive tests might need confirmation by culture or PCR. We explored whether DNA from residual faecal matter on rapid diagnostic tests could be extracted for PCR confirmation.

Results

Conclusion