Chokeberry Extract and Its Active Polyphenols Suppress Adipogenesis in 3T3-L1 Adipocytes and Modulates Fat Accumulation and Insulin Resistance in Diet-Induced Obese Mice.

Na-Hyun Kim,Yun Kim,Eun Jeong,Jung-Rae Rho,Jonghwan Jegal,Min Yang,Jeong-Doo Heo

doi:10.3390/nu10111734

Abstract

Berries of Aronia melanocarpa (chokeberry) are known to be a rich source of biologically active polyphenols. In the present study, the effects of seven anti-adipogenic polyphenolic phytochemicals isolated from A. melanocarpa methanol extract on adipogenic transcription factors were investigated. Amygdalin and prunasin were found to inhibit 3T3-L1 adipocyte differentiation by suppressing the expressions of PPARγ (peroxisome proliferator-activated receptor γ), C/EBPα (CCAAT/enhancer binding protein α), SREBP1c (sterol regulatory element binding protein 1c), FAS (fatty acid synthase), and aP2 (adipocyte fatty-acid–binding protein). A. melanocarpa extract-treated (100 or 200 mg/kg/day on body weight) high fat diet (HFD)-induced obese mice showed significant decreases in body weight, serum triglyceride (TG), and low-density lipoprotein cholesterol (LDLC) levels and improved insulin sensitivity as compared with HFD controls. This research shows A. melanocarpa extract is potentially beneficial for the suppression of HFD-induced obesity.

Highlights

Obesity is a chronic, relapsing, and multifactorial disorder with wide-ranging causes [1,2], and in Asian men is defined as a body mass index (BMI) of ≥30 kg/m2 [3]
An in vitro study was undertaken to investigate the effects of compounds 1–7 on adipogenic transcription factors in 3T3-L1 adipocytes
The present work provides direct evidence of the beneficial effects of A. melanocarpa extract on diet-induced obesity in mice

Summary

Introduction

Obesity is a chronic, relapsing, and multifactorial disorder with wide-ranging causes [1,2], and in Asian men is defined as a body mass index (BMI) of ≥30 kg/m2 [3]. Obesity is known to be associated with diverse comorbidities including cardiovascular diseases, dyslipidemia, and type 2 diabetes [4]. Today obesity has reached epidemic proportions, and has become a major global public health problem. Adipocyte growth occurs due to hypertrophy (an increase in cell size) and hyperplasia (an increase in cell number) [5,6], and white adipose tissue acts as a major endocrine organ by secreting protein signals and factors called adipokines [7]. The precise mechanism has not been delineated, it is generally held that the pathogeneses of complications related to obesity involve abnormal adipokine production by adipocytes [8]

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Results

Conclusion