Choice for host-specific high-adhesive &amp;lt;i&amp;gt;Lactobacillus&amp;lt;/i&amp;gt; strains

Xueyan Lin,Hongjie Liu,Zhongxiang Niu,Zhonghua Wang,Yun Wang

doi:10.4236/abb.2012.32022

Abstract

Adhesive ability was tested in seven Lactobacillus solated from the chicken digestive tract after cultivation with CaCo-2 cells (intestinal epithelial cells), MDCK (dog kidney) cells and CEF (chicken embryo fiber) cells. We noted the following important observations regarding the adhesive ability between different Lactobacillus strains and three cell types: the adhesive interaction between the SDnB7, SDnE1 and SDnA3 lactobacillus strains and CaCo-2cells was greater compared to controls, the adhesive effect between SDnB1 and CEF cells was also greater than controls and Lactobacillus showed only minimal adherence to MDCK cells. Incubation time also affected Lactobacillus adherence to CaCo-2cells: adhesive ability was optimal at 37°C when incubated for 2 days and this was confirmed by a local increase in the concentration of Lactobacillus around CaCo-2 cells when incubated for 24 h as opposed to 3 h. Adherence ability in lactobacillus was also tested at various concentrations (108, 107, 106, 105 and 104 ). The number of Lactobacillus that adhered around the cells was significantly increased in the treatment with 108 bacterial cells. Transmission electron microscopy revealed that the cellularity of the junction between CaCo-2cells and Lactobacillus was not compromised. Transmission electron microscopy also revealed that the thalli fabric structure remained intact.

Highlights

The definition for probiotics has gradually changed with an increasing understanding of how it influences human and animal health
We noted the following important observations regarding the adhesive ability between different lactobacillus strains and three cell types: the adhesive interaction between the SDnB7, SDnE1 and SDnA3 lactobacillus strains and CaCo-2 cells was greater compared to controls, the adhesive effect between SDnB1 and CEF cells was greater than controls and lactobacillus showed only minimal adherence to MDCK cells
Incubation time affected lactobacillus adherence to CaCo-2 cells: adhesive ability was optimal at 37 ̊C when incubated for 2 days and this was confirmed by a local increase in the concentration of lactobacillus around CaCo-2 cells when incubated for 24 h as opposed to 3 h

Summary

Introduction

The definition for probiotics has gradually changed with an increasing understanding of how it influences human and animal health. A potentially successful probiotic strain is expected to have several desirable properties in order to show benefit. These properties include the ability to adhere to cells while remaining non-pathogenic. Adherence to the intestinal mucosa is considered selec-. Lactobacillus bacteria have the capacity to adhere to intestinal epithelial cells. Different species of these bacterium vary in their adherence ability. Rinkinen (2003) and Gusils (2002) suggested that the adhesive ability of probiotic strains is species specific but not host-specific [12,13]. On the other hand, aimed to demonstrate that strains were species-specific and hostspecific

Methods

Results

Conclusion