Abstract
Biopolymer based nanoformulation was synthesized by using microwave assisted nano-precipitaion method. The biopolymers consist of chitosan and guar gum were crosslinked using citric acid in order to encapsulate chlorpyrifos pesticide. The successful synthesis of chlorpyrifos containing nano-formulations was thoroughly examined, where the surface morphology examined by using field emission scanning electron microscopy (FE-SEM) has established the loading of chlorpyrifos in the biopolymeric matrix, transmission electron microscopy (TEM) examination revealed the spherical shaped particles of about 234 nm and Fourier-transform infrared spectroscopy (FTIR) analysis has confirmed the crosslinking between two biopolymers through citric acid due to the presence of peaks corresponding to ester linkages at 1730 cm-1. The encapsulation efficiency of chlorpyrifos at pH 7 and 30 ºC was around 50%. The successfully synthesized chlorpyrifos loaded biopolymeric nano-formulation were further utilized to study the release behaviour of chlorpyrifos in water and biocompatibility towards soil microbiota. The release of chlorpyrifos was almost 15% slower than conventional chlorpyrifos and the formulation was found biocompatible towards microbiota.
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