Chlorpyrifos levels within permitted limits induce nuclear abnormalities and DNA damage in the erythrocytes of the common carp.

Abstract

The organophosphate pesticide chlorpyrifos (CPF) is defined as a priority pollutant in surface freshwaters according to Directive 2013/39/EU of the European Parliament. The focus of this study was to assess the potential cytotoxic and genotoxic effects of permissible CPF levels on juvenile forms of the common carp. We found that low-level CPF exposure did not induce elevated levels of micronuclei, but significantly increased the frequency of total nuclear abnormalities (NAs) proportional to dose and time; notched, blebbed, lobed and eight-shaped nuclei, nuclear buds, nuclear bridges and binucleated cells were all detected. Decreased frequencies of polychromatic erythrocytes (PCEs) and DNA damage detected by comet assay were also observed, confirming the cytotoxic and genotoxic effects of CPF. Altogether, these data (1) demonstrate that CPF is toxic even at permissible levels, possessing considerable genotoxic and cytotoxic potential in peripheral erythrocytes of exposed fish and (2) validate the assessment of NAs, PCEs and comet assay performance as sensitive biomarkers for the early detection of CPF pollution. These findings can be applied to guide environmental risk assessment and biomonitoring programs.