Abstract
We reported a one-pot, environmentally friendly method for biosynthesizing nanoscale Au-Ag alloy using chloroplasts as reducers and stabilizers. The prepared nanoscale Au-Ag alloy was characterized by UV–visible spectroscopy, X-ray diffraction (XRD) and high resolution transmission electron microscopy (HR-TEM). Fourier transform infrared spectroscopy (FTIR) analysis was further used to identify the possible biomolecules from chloroplasts that are responsible for the formation and stabilization of Au-Ag alloy. The FTIR results showed that chloroplast proteins bound to the nanoscale Au-Ag alloy through free amino groups. The bimetallic Au-Ag nanoparticles have only one plasmon band, indicating the formation of an alloy structure. HR-TEM images showed that the prepared Au-Ag alloy was spherical and 15 to 20 nm in diameter. The high crystallinity of the Au-Ag alloy was confirmed by SAED and XRD patterns. The prepared Au-Ag alloy was dispersed into multiwalled carbon nanotubes (MWNTs) to form a nanosensing film. The nanosensing film exhibited high electrocatalytic activity for 2-butanone oxidation at room temperature. The anodic peak current (Ip) has a linear relationship with the concentrations of 2-butanone over the range of 0.01% to 0.075% (v/v), when analyzed by cyclic voltammetry. The excellent electronic catalytic characteristics might be attributed to the synergistic electron transfer effects of Au-Ag alloy and MWNTs. It can reasonably be expected that this electrochemical biosensor provided a promising platform for developing a breath sensor to screen and pre-warn of early cancer, especially gastric cancer.
Highlights
Bimetallic nanoparticles display different catalytic properties, surface energy and magnetic properties depending on the different types of metal nanoparticles [1,2,3,4]
We report the biosynthesis of nanoscale Au-Ag alloy in chloroplasts solution and further test the possibility to use Au-Ag alloy as an electrochemical biosensor
We investigated the electrocatalytic properties of the Au-Ag/multiwalled carbon nanotubes (MWNTs) nanosensing film for low concentration of 2-butanone
Summary
Bimetallic nanoparticles display different catalytic properties, surface energy and magnetic properties depending on the different types of metal nanoparticles [1,2,3,4]. Au– Ag bimetallic nanoparticles attract great attention due to their unique optical, electrochemical properties and important applications as biosensors [5,6,7,8]. Microorganisms and extracts of plants have been used for Electrochemical biosensors have been one of the powerful tools for cancer diagnostics, as a simple-preparation, low-cost, rapid-response and portable platform [14,15,16]. The coupling of electrochemical devices with nanomaterials offers a unique capability for accurate measurements of multiple cancer markers from a variety of cancer patient samples. Au-containing bimetallic nanoparticles have been expected to enhance the catalytic activity and selectivity of electrochemical sensors [17,18,19,20]
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