Abstract
Malic dehydrogenase (MDH) isozymes are well known in plant tissues. Observations on the intracellular localization of dehydrogenase ( Price and Thimann, 1954) has prompted detailed studies of malic dehydrogenase isozymes. Because malic dehydrogenase activity has been reported in the chloroplasts of higher plants (Pierpoint, 1963), we hypothesized that chloroplasts would have a unique malic dehydrogenase protein. Schweiger, Master, and Werz (1967) reported that the main two MDH isozymes in Acetabularia mediterranea were associated with chloroplasts. In this report, evidence is presented for a new malic dehydrogenase isozyme which we have designated as chloroplast-malic dehydrogenase.
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