Abstract
1. Leaves of Spinacia oleracea have the typical structure of mesophytic, dicotyledonous leaves. 2. Pigmented granules, which are distinct morphological structures and resemble the grana of mesophyll chloroplasts, are present in the cytoplasm of both upper and lower epidermal cells of spinach leaves. 3. These granules are of two types: light-green, an average of 1.1 μ in diameter; and yellow-green, an average of 1.2 μ in diameter. Both types demonstrate fluorescence and birefringence; and, therefore, they may be chlorophyll-containing structures. They may possibly be either immature or degenerate chloroplasts. 4. Glycerine and formalin treatment of the epidermal tissues does not affect the birefringence of the granules even after 12 days, though after 30 days in glycerine their birefringence may be lost.
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