Abstract
The restriction enzyme cleavage patterns of chloroplast DNA of eleven diploid species of Brassica and its allied genera were compared by using five restriction endonucleases. A dendrogram of species relationships as shown by the estimated numbers of recognition site mutations was drawn, the species being grouped into three clusters at the 5 recognition site mutations. The clusters did not always correspond to genera recognized in current taxonomy, while good correspondence was observed with the grouping by fraction I protein large subunits. The species relationships as seen with the present method were significantly correlated with those found in cytogenetical studies but not with those based on numerical morphological taxonomy.
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