Abstract
We cloned all of Adiantum capillus-veneris chloroplast DNA PstI fragments longer than 1.0 kb, which cover 98% of the genome. These cloned fragments were used to construct a physical map for five restriction enzymes. The genome of A. capillus-veneris is approximately 153 kb long and contains a 24 kb inverted repeat. Mapping of 12 chloroplast DNA genes and heterologous hybridization, involving A. capillus-veneris chloroplast DNA and angiosperm chloroplast DNA probes, demonstrated that chloroplast DNA of A. capillus-veneris has a different gene order from typical angiosperm cpDNA (e.g., tobacco) in the inverted repeat region and the flanking segment of the large single copy region.
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