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Abstract
The conversion of divinyl protochlorophyllide a (DV Pchlide a) (E443 F625), where E and F refer to fluorescence excitation and emission maxima respectively in ether at 77 K, into chlorophyllide a (Chlide a) (E459 F675) is reported. This reaction was demonstrated by first inducing etiolated cucumber cotyledons to accumulate DV Pchlide a (E443 F625) devoid of monovinyl (MV) contamination by a series of light/dark treatments. The accumulated DV Pchlide a (E443 F625) was then converted into Chlide a (E459 F675) by a 2.5-ms light pulse (L). The Chlide a (E459 F675) thus generated exhibited spectrofluorometric properties which differed from those of MV Chlide a (E447 F674) at 77 K and at room temperature before and after demetallation. The techniques described in this work permit the preparation of Chlide a (E459 F675) devoid of MV Chlide a contamination, for further chemical structural identification of this novel chlorophyll (Chl) precursor.
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