Abstract
Tissue culturing is widely used for the clonal propagation of several plant species. However, high mortality rates may occur during the acclimatization phase due to the sudden, dramatic changes in light levels. This study evaluated the effect of light level during the acclimatization of Neoregelia concentrica (Vellozo) LB Smith using chlorophyll a fluorescence and growth analysis. N. concentrica plants were multiplied and rooted in vitro on Murashige and Skoog medium and then transferred to plastic trays with individual cells containing vermiculite. The treatments consisted of the ex vitro cultivation of plants under light levels of 30, 50, 70, and full sunlight (100% light) for 28 d. Chlorophyll a fluorescence measurements were taken at 0 (in vitro), 7, 14, 21, and 28 d of acclimatization, and growth trait analyses were made at 28 d. The high irradiance under full sunlight (100% light) induced photoinhibition and reduced the growth rate. Plants grown under partial light (30, 50, and 70%) were less inhibited. A 50% reduction in light level during N. concentrica acclimatization produced rapid plant recovery and higher growth rates than full sunlight.
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