Abstract
The influence of alachlor and metazachlor on fatty acid synthesis of the green alga Scenedesmus acutus has been investigated. Autotrophic growth for 40 hr in the presence of 5 μ M alachlor or metazachlor led to a strong decrease in linolenic acid (18:3) content, while an increase in the palmitic (16:0) and oleic acid (18:1) content was observed. Furthermore, short-term incorporation studies (3 hr) with [2- 14C]acetate as the precursor of fatty acids showed a substantial inhibition of acetate incorporation into the fatty acid extract. Using TLC and HPLC techniques this extract exhibited one 14C-labeled fraction. As analyzed by gas chromatography linolenic acid is the main component in this fraction (more than 30%). A 5-μ M and 10-μ M sethoxydim application had no effect on acetate uptake with Scenedesmus. A combination of either metazachlor or alachlor with sethoxydim markedly improved the inhibitory activity of the chloroacetamides on acetate uptake. Our data suggest an inhibition of fatty acid biosynthesis between the C-elongation step of palmitic acid and the desaturation of oleic acid yielding linolenic acid.
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