Abstract
Chemical investigation of the tunicate Trididemnum solidum resulted in the isolation of two new chlorinated compounds belonging to the didemnin class, along with two known compounds didemnin A and didemnin B. The structural determination of the compounds was based on extensive NMR and mass spectroscopic analysis. The isolated compounds 1–4 were tested for their anti-inflammatory activity using in vitro assays for inhibition of inducible nitric oxide synthase (iNOS) and nuclear factor-kappa B (NF-κB) activity. The anti-cell proliferative activity of the above compounds was also evaluated against four solid tumor cell lines.
Highlights
Didemnins belonging to the class of depsipeptides were first isolated from the Caribbean tunicateTrididemnum solidum (Family Didemnidae) [1,2]
The crude extract was further subjected to a series of chromatographic separations to yield the didemnin class of pure compounds (1–4), and the isolated compounds were tested for their in vitro anti-inflammatory and anticancer activities
Inhibition of inducible nitric oxide synthase (iNOS) activity was determined in RAW264.7 cells as described earlier [22]
Summary
Trididemnum solidum (Family Didemnidae) [1,2]. Didemnin B, a potent anticancer agent was the first marine natural product that entered into phase I clinical trials. Didemnin B exhibited a variety of biological activities which includes inhibition of the growth of both RNA and DNA viruses [3], in vitro and in vivo activity against B16 melanoma, in vivo activity against P388 leukemia and in vitro. In the course of our search to identify new drug candidates and targets, the tunicate Trididemnum solidum (KY10508001) was collected from the Little. The extract was found to exhibit strong anti-inflammatory activity using in vitro assays for inhibition of inducible nitric oxide synthase (iNOS) and nuclear factor-kappa B (NF-κB) activity. The crude extract was further subjected to a series of chromatographic separations to yield the didemnin class of pure compounds (1–4), and the isolated compounds were tested for their in vitro anti-inflammatory and anticancer activities
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