Abstract
The plasma-induced instability of reverse-phase evaporation vesicles (REV) was successfully evaluated by measuring chloride leakage. The chloride efflux could be followed by using a silver chloride electrode without interference from coexisting substances such as liposomal lipid, plasma or bovine serum albumin (BSA). The permeability of REV was increased by incubation with human plasma or BSA ; the effect of plasma was estimated to be approximately 50 times larger than that of a corresponding amount of albumin. The difference of protein-lipid interaction in the cases of plasma and isolated albumin is discussed on the basis of the leakage pattern and the activation energy of the permeation process.
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