Chloride, Gluconate, Sulfate, and Short-Chain Fatty Acids Affect Calcium Flux Rates Across the Sheep Forestomach Epithelium

Abstract

In ruminants, more than 50% of overall gastrointestinal Ca absorption can occur preintestinally, and the anions of orally applied Ca salts are thought to play an important role in stimulating ruminal Ca absorption. This assumption is based mainly on ion-exchange studies that have used gluconate as the control anion, which may bind Ca2+ ions and interfere with treatment effects. In the present study, we investigated the distinct effects of different anions on Ca absorption across the sheep rumen and on the concentration of free Ca2+ ions ([Ca2+]ion). We showed that gluconate, sulfate, and short-chain fatty acids (SCFA) remarkably reduced [Ca2+]ion in buffer solutions. Nevertheless, increasing the Cl or SCFA concentration by 60mM stimulated net ruminal Ca absorption 5- to 7-fold, but these effects could be antagonized by gluconate. Therefore, ion-exchange experiments must be (re)evaluated very carefully, because changes in [Ca2+]ion in the presence of gluconate, sulfate, or SCFA not only might entail an underestimation of Ca flux rates, but also might have effects on other cellular pathways that are Ca2+ dependent. Concerning the optimal Ca supply for dairy cows, the present study suggests that CaCl2 formulations and Ca salts of the SCFA stimulate Ca absorption across the rumen wall and are beneficial in preventing or correcting a Ca deficiency.