Chlorhexidine versus organoselenium for inhibition of S. mutans biofilm, an in vitro study

Abbas Zaher,Mohamed Mowafy,Ahmed Gaballah,Abdul Seguya

doi:10.1186/s12903-022-02049-w

Abstract

BackgroundChemical Plaque control by antimicrobial agent application can defend the teeth against caries. S. mutans is considered the main etiologic factor for caries. This was an in vitro study to compare between the efficacy of chlorhexidine diaceteate varnish, and an organoselenium sealant, to prevent S. mutans biofilm formation on human teeth.MethodsFourty five premolars extracted for orthodontic purposes were randomly divided into 3 groups of 15 teeth each. One control group and two test groups, chlorhexidine diaceteate varnish and an organoselenium sealant. The teeth were autoclaved before S. mutans biofilm was induced on to each in their respective groups. The reading T1 was taken for each tooth to assess the number of S. mutans attached in order to compare for differences in surface area among the 3 groups. The respective test materials were applied onto the teeth and biofilm induced onto them in their respective groups. The reading T2 was taken for the 2 test groups. The 3 groups were then subjected to aging for a period equivalent to 5 months before the biofilm was induced to take the reading T3 for the number of S. mutans. We used vortexing of the teeth to disrupt the biofilm at time points T1, T2 and T3. S. mutans count was then done using PCR.ResultsThere were significantly lower S. mutans counts in the control group as compared to the chlorhexidine diacetate group at T3.There were no other statistically significant differences found.ConclusionBoth organoselenium and Chlorhexidine diacetate do not inhibit S. mutans biofilm attachment onto the teeth.

Highlights

The oral cavity has about 700 known species of bacteria, including 140 dominating ones that make the multispecies biofilm, dental plaque [1]
There have been in vivo studies investigating plaque formation around Organoselenium sealants but these were using a multiorganism vehicle
The aim of this work is to compare the efficacy of a chlorhexidine containing varnish (Cervitec Plus), and a selenium containing sealant (DenteShieldTM) in the inhibition of biofilm formation on tooth surfaces immediately after application of the materials and 5 months after aging factor. It is a randomized controlled in-vitro study to compare between the efficacy of the application of a chlorhexidine diacetate varnish and that of a selenium containing sealant (DenteShieldTM)in the prevention of biofilm formation on teeth

Summary

Introduction

The oral cavity has about 700 known species of bacteria, including 140 dominating ones that make the multispecies biofilm, dental plaque [1]. By antimicrobial agents application, can defend the teeth against negative effects, to reduce the accumulation of plaque, and to better oral health [6,7,8]. White Spot Lesions (WSLs) are a significant clinical problem in relation to treatment with fixed orthodontic appliances [9]. WSLs can be seen within 4 weeks after fixed orthodontic appliances treatment commencement [11], if preventive measures are not established [12]. Chemical Plaque control by antimicrobial agent application can defend the teeth against caries. S. mutans is considered the main etiologic factor for caries. This was an in vitro study to compare between the efficacy of chlorhexidine diaceteate varnish, and an organoselenium sealant, to prevent S. mutans biofilm formation on human teeth

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