Abstract

녹색 미세조류인 Chlorella sp.를 사용하여 광도에 따른 미세조류의 비성정속도와 광합성을 측정하였고, 여러 가지 kinetic model을 이용해 실측된 자료에 가장 적합한 kinetic model을 모색하였다. Chlorella sp.는 광독립영양조건 (photoautotroph condition)과 27, 54, 81, 140, 281 <TEX>${\mu}mol/m^2{\cdot}s$</TEX>의 광도 하에서 배양되었으며 kinetic model을 사용하여 P-I 반응선도와 <TEX>${\mu}-I$</TEX> 반응선도를 구하였다. Chlorella sp.의 비성장속도와 광합성은 81 <TEX>${\mu}mol/m^2{\cdot}s$</TEX>에서 0.35/day, 0.94 <TEX>$mgO_2/g{\cdot}h$</TEX>로 가장 높았다. 실측된 자료를 기질속도론 (Tamiya), 광포화모형(hyperbolic tangent, 포아송 함수)과 두가지 광저해모형(Steel 지수함수, Andrew 기질저해함수)을 이용하여 그게 따른 계수를 추정하고 비교 분석하였다. <TEX>${\mu}-I$</TEX> 반응선도에서는 포화모형의 일종인 hyperbolic tangent 모형이 적합하였으며, P-I 반응선도에서는 광저해가 현저히 관찰되어 Steele 지수함수의 적합도가 우수하였다. We investigated which is the best kinetic model for expression of microalgal growth and photosynthesis by irradiance in green microalgae, marine Chlorella sp. is cultivated under 5 light intensities (27, 54, 81, 140, and 281 <TEX>${\mu}mol/m^2{\cdot}s$</TEX>). The maximum of specific growth rate and photosynthesis at light intensity of 81 <TEX>${\mu}mol/m^2{\cdot}s$</TEX> were observed as 0.35/day and 0.94 <TEX>$mg0_2/g{\cdot}h$</TEX>, respectively. To describe the effect of irradiance, we adapted both types for kinetic model fitting, saturation and inhibition models, Tamiya's modified Michaelis- Menten equation, hyperbolic tangent and Poisson function as saturation model, Steele's exponential and Andrew's function as the other and made the specific growth rate- irradiance response curve and photosynthesis- irradiance response curve. In case of microalgal growth, one of the saturation models, the hyperbolic tangent function was the most suitable, otherwise, the Steele's exponential function, one of the inhibition models, was suitable for photosynthesis.

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