Chlamydospore Specific Proteins of Candida albicans

Sujata Ingle,Santosh Kodgire,Asha Shiradhone,Gajanan Zore,Rajendra Patil

doi:10.3390/data2030026

Sujata Ingle, Santosh Kodgire + Show 3 more

Open Access

https://doi.org/10.3390/data2030026

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Abstract

Polymorphic yeast, Candida albicans, forms thick-walled structures called chlamydospores in order to survive under adverse conditions. We present proteomic profile changes occurring during chlamydospore formation. Chlamydospores were induced by inoculating C. albicans cells (grown for 48 h) on rice extract and semisolid agar containing tween 80 (1%), and were overlaid by a polyethene sheet to induce microaerophilic conditions at 30 °C. Proteins extracted from chlamydospores and hyphae (producing chlamydospores) were identified by LC-MS/MS analysis. Present datasets include proteomic data (Swath spectral libraries) of chlamydospores and yeast phase cells, as well as methodologies and tools used for the data generation. Further analysis is expected to provide an opportunity to understand modulations in metabolic processes, molecular architecture (i.e., cell wall, membrane, and cytoskeleton) and stress response pathways leading to chlamydospore formation and thus facilitating survival of C. albicans under adverse conditions.

Highlights

Morphophysiological plasticity enables Candida albicans to survive under a variety of extreme microenvironments [1,2,3,4,5,6,7,8,9,10,11]
C. albicans through processed peptides using generated through processed peptides using Micro LC-Triple TOF 5600 SWATH MS analysis
Among these[23], morphological forms, chlamydospores

Summary

Introduction

Morphophysiological plasticity enables Candida albicans to survive under a variety of extreme microenvironments [1,2,3,4,5,6,7,8,9,10,11] It exists in the form of yeast, hyphae, pseudohyphae, chlamydospore, white/opaque cells, according to the microenvironment [1,2,3,4,5,6,7,8,9,10,11]. Cells in different morphological forms respond differently towards host defense mechanisms, antifungal agents, stress conditions [8] It makes C. albicans one of the most successful opportunistic pathogens of humans [1,2]. Among these morphological forms, chlamydospore is one of the most interesting structures, and develops under unfavorable conditions such as oxygen limitation and embedded growth in matrix (Figure 1a) [5,6,7].

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