Chlamydia trachomatis induces autophagy by p62 in HeLa cell.

Abstract

Chlamydia trachomatis is the most common bacterial pathogen causing sexually transmitted diseases. C. trachomatis infection is closely related to the development of cervical cancer, studies have shown that C. trachomatis can induce host cell autophagy. The autophagy related gene p62 plays an important role in the process of autophagy. To further understand the role of autophagy-associated gene p62 in autophagy of HeLa cells induced by C. trachomatis, p62-silencing cell line, HeLa229-shp62, and control cell line, HeLa229-shNC, were constructed, and a C. trachomatis-infected cell model was established. The autophagosome and C. trachomatis inclusions were observed under electron microscope. The autophagy level of C. trachomatis-infected HeLa cells was detected by Western blot. The results suggested that knockdown of p62 affected neither C. trachomatis infection of HeLa cells nor the initiation of C. trachomatis-induced autophagy, but at 48h post C. trachomatis infection, autophagy levels were significantly inhibited in p62 silencing host cells. The study demonstrated the important role of p62 in the autophagy induced by C. trachomatis in HeLa cells, which could provide data support and theoretical basis for exploring the pathogenesis and prevention of C. trachomatis.