Abstract
Chitosan-coated magnetic nanoparticles (MNPs@CTS) which used as substrate immobilization carrier of glucosyl acceptor for α-arbutin production is introduced in this study. Fe3O4@CTS microspheres were prepared via an oxidation hydrothermal method, and further modified with chitosan. Substrate hydroquinone (HQ) was immobilized on the Fe3O4@CTS. When 1.0 g/L Fe3O4@CTS was shaken in HQ solution (7.0 g/L) at 28 °C with 120 rpm for 12 h, the maximum amount of HQ immobilization on the Fe3O4@CTS could reach 312 mg HQ per gram Fe3O4@CTS. The optimal reaction conditions for α-arbutin synthesis were also investigated. When 25 g/L Fe3O4@CTS@HQ (70.9 mM HQ) was shaken in fermentation broth at 30 °C with 180 rpm for 40 h, the final yield of α-arbutin reached 7.85 g/L. This work is useful for the industrial-scale production of α-arbutin.
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