Chitosan-Based Thermo-Sensitive Hydrogel Loading Oyster Peptides for Hemostasis Application.

Dongying Zhang,Lingyu Zhang,Sitong Lu,Sidong Li,Zhang Hu,Fengyan Liang

doi:10.3390/ma13215038

Abstract

Uncontrolled massive hemorrhage is one of the principal causes of death in trauma emergencies. By using catechol-modified chitosan (CS-C) as the matrix material and β glycerol phosphate (β-GP) as a thermo-sensitive agent, chitosan-based thermo-sensitive hydrogel loading oyster peptides (CS-C/OP/β-GP) were prepared at physiological temperature. The hemostatic performance of CS-C/OP/β-GP hydrogel was tested in vivo and in vitro, and its biological safety was evaluated. The results showed that the in vitro coagulation time and blood coagulation index of CS-C/OP/β-GP hydrogel were better than those of a commercial gelatin sponge. Notably, compared with the gelatin sponge, CS-C/OP/β-GP hydrogel showed that the platelet adhesion and erythrocyte adsorption rates were 38.98% and 95.87% higher, respectively. Additionally, the hemostasis time in mouse liver injury was shortened by 19.5%, and the mass of blood loss in the mouse tail amputation model was reduced by 18.9%. The safety evaluation results demonstrated that CS-C/OP/β-GP had no cytotoxicity to L929 cells, and the hemolysis rates were less than 5% within 1 mg/mL, suggesting good biocompatibility. In conclusion, our results indicate that CS-C/OP/β-GP is expected to be a promising dressing in the field of medical hemostasis.

Highlights

Rapid hemostasis in emergencies has great clinical significance for patients with massive and potentially fatal hemorrhage caused by war, operations, accidents, or hematologic disease [1].Hemostasis is an important step in emergency medical treatment [2]
While they have obvious hemostatic effects, their shortcomings are considerable; for example, collagen dressings have poor tissue adhesion [3]; porous zeolite releases heat after absorbing water in the blood, which may lead to wound inflammation [4]; and carboxymethyl gauze cannot be degraded on the wound surface, which can create scars following removal [5]
Scanningelectron electron microscope (SEM) revealed that the hydrogel CS-C/Oyster peptides (OP)/β glycerol phosphate (β-GP) had an even porous network structure similar to the commercial gelatin sponge with an average pore size of 100–200 μm (Figure 1)

Summary

Introduction

Rapid hemostasis in emergencies has great clinical significance for patients with massive and potentially fatal hemorrhage caused by war, operations, accidents, or hematologic disease [1].Hemostasis is an important step in emergency medical treatment [2]. Many kinds of hemostatic materials are available on the market While they have obvious hemostatic effects, their shortcomings are considerable; for example, collagen dressings have poor tissue adhesion [3]; porous zeolite releases heat after absorbing water in the blood, which may lead to wound inflammation [4]; and carboxymethyl gauze cannot be degraded on the wound surface, which can create scars following removal [5]. As a natural polycation polysaccharide prepared by chitin deacetylation, chitosan is characterized by excellent coagulation, bacteriostasis, a film-forming ability, wound healing promotion, and scar formation inhibition. In addition to these features, it is a multifunctional material with good biocompatibility, no immunogenicity, and no dermal irritation [6,7,8].

Methods

Results

Conclusion