Chitosan-Zn Chelate Increases Antioxidant Enzyme Activity and Improves Immune Function in Weaned Piglets

Abstract

This experiment was performed in order to investigate the effects of chitosan-Zn chelate (CS-Zn) on activities of antioxidant enzymes and immune function in weaned piglets. One hundred and twenty weaned piglets (Duroc × Landrace × Yorkshire) with 7.12 ± 0.25 kg body weight were allotted to four treatments. A basal diet without Zn supplementation was used as control group. The other three treatments were fed the control diet supplemented with 100 mg/kg Zn as ZnSO4, 100 mg/kg Zn as CS-Zn, 100 mg/kg Zn as ZnSO4 and chitosan (the content of chitosan was the same as that of CS-Zn), respectively. The feeding trial lasted 30 days. Spleen index of pigs fed dietary CS-Zn was higher (p < 0.05) than that of control pigs. Thymus index and lymph node index did not differ among the pigs fed any diets (p > 0.05). T-AOC levels, Cu-ZnSOD, and GSH-PX activities in serum or liver of the pigs receiving CS-Zn diet were higher than those of the pigs fed CS+ZnSO4 or ZnSO4 diets (p < 0.05). These pigs fed dietary CS-Zn also showed lower MDA content in liver compared with the pigs fed other diets (p < 0.05). Serum IgA, complement 3, and complement 4 levels of pig fed dietary CS-Zn was higher than those of the pigs fed other diets (p < 0.05). Supplemental dietary Zn did not change serum IgG and IgM levels (p > 0.05). The ALP activity of pigs fed dietary CS-Zn was higher than those of the pigs fed other three diets (p < 0.05). No significant differences were founded in serum GOT or GPT activities of pigs fed dietary Zn (p > 0.05). The results of the present study indicated that chitosan-Zn chelate could increase antioxidant capacity and improve immune function in weaned piglets compared with ZnSO4 or chitosan.