Abstract
In the current research, to find if the combination of chitosan nerve conduits seeded with autologous bone marrow mononuclear cells (BM-MNCs) can be used to bridge 30 mm long peroneal nerve defects in goats, 15 animals were separated into BM-MNC group (n = 5), vehicle group (n = 5), and autologous nerve graft group (n = 5). 12 months after the surgery, animals were evaluated by behavioral observation, magnetic resonance imaging tests, histomorphological and electrophysiological analysis. Results revealed that animals in BM-MNC group and autologous nerve graft group achieved fine functional recovery; magnetic resonance imaging tests and histomorphometry analysis showed that the nerve defect was bridged by myelinated nerve axons in those animals. No significant difference was found between the two groups concerning myelinated axon density, axon diameter, myelin sheath thickness and peroneal nerve action potential. Animals in vehicle group failed to achieve significant functional recovery. The results indicated that chitosan nerve conduits seeded with autologous bone marrow mononuclear cells have strong potential in bridging long peripheral nerve defects and could be applied in future clinical trials.
Highlights
ObjectivesA combination of chitosan nerve conduits and certain other materials might result in compounds with unique abilities, since the purpose of the current research was to test the potential of clinical translation of this material, we have applied chitosan conduits only in this study
Carried out to test the curative effect of chitosan tubes seeded with autologous BM-MNCs in repairing 30 mm goat common peroneal nerve defect
No skin ulcer or obvious dystrophy of anterior tibial muscle was detected in animals from BM-MNC group and nerve graft group
Summary
A combination of chitosan nerve conduits and certain other materials might result in compounds with unique abilities, since the purpose of the current research was to test the potential of clinical translation of this material, we have applied chitosan conduits only in this study. Since the main purpose of this research was to find if the combined application of chitosan nerve tubes and mononuclear stem cells can have ideal long term effect in bridging long peroneal nerves
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