Abstract
In this study, cisplatin (CDDP)-loaded chitosan-coated solid lipid nanoparticles (SLN) was successfully formulated to treat HeLa cervical carcinoma. The formulation nanoparticles were nanosized and exhibited a controlled release of drug in physiological conditions. The blank nanoparticles exhibited an excellent biocompatibility profile indicating its suitability for cancer targeting. The incorporation of CDDP in SLN remarkably increased the cancer cell death as evident from the MTT assay. Importantly, CDDP-loaded chitosan-coated SLN (CChSLN) significantly (P < 0.05) decreased the viability of cancer cells even at low concentration. The higher cytotoxicity potential of CChSLN was attributed to the higher cellular uptake as well as the sustained drug release manner in comparison with CSLN. Consistent with the cytotoxicity assay, CChSLN showed the lowest IC50 value of 0.6125 μg/ml while CSLN presented 1.156 μg/ml. CChSLN showed a significantly higher apoptosis in cancer cells compared to that of CSLN and CDDP, which is attributed to the better internalization of nanocarriers and controlled release of anticancer drugs in the intracellular environment. Our findings suggest that this new formulation could be a promising alternative for the treatment of cervical cancers. These findings are encouraging us to continue our research, with a more extended investigation of cellular response in real time and in animal models.
Highlights
Human cervical cancer is one of the popular cancers in women in reproductive age across the globe [1]
Preparation of Cisplatin-Loaded Chitosan-Coated Solid Lipid Nanoparticles The solid lipid nanoparticles (SLN) was formulated by hot homogenization followed by emulsification-ultrasound method
Physicochemical Characterization of CChSLN SLN was prepared by hot homogenization followed by emulsification-ultrasound method
Summary
Human cervical cancer is one of the popular cancers in women in reproductive age across the globe [1]. The cervical cancer is caused by human papillomavirus (HPV). International Agency for Research on Cancer has stated the main factors responsible for the cervical cancers including viral infection, inordinate sexual behavior, depleted immune system, and smoking [2]. Recent advances in cancer diagnosis and use of vaccine have reduced the mortality rate of cervical cancer patients; cancer-related death in China is still high. Two prophylactic HPV vaccines (Gardasil and Cervarix) were marketed to tackle HPV-associated cervical cancers; it was effective only in adult patients and failed to show any therapeutic effect against present cases. In the clinical settings, chemotherapeutic drugs play an important role in killing the cervical cancer cells [4, 5]
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