Chitosan/gum tragacanth/PVA hybrid nanofibrous scaffold for tissue engineering applications

Abstract

In this paper, preparation and characterization of a nanofibrous scaffold comprising both chitosan (CS) and gum tragacanth (GT) are reported for the first time. CS and GT were separately blended with poly(vinyl alcohol) (PVA) and simultaneously electrospun by a double-nozzle electrospinning apparatus, and the resulting nanofibrous mats were compared with CS/PVA and GT/PVA electrospun ones. Scanning electron microscopy images showed uniform bead-free nanofibers with some merging areas and an average fiber diameter of ∼273 nm for CS-PVA/GT-PVA. The ultimate tensile strength and strain at break of the hybrid nanofibers were ∼20 MPa and ∼9%, respectively, which were significantly higher than those of the CS/PVA and GT/PVA nanofibers. The CS-PVA/GT-PVA nanofibrous mats also showed a water droplet contact angle value (∼31°) between those of the CS/PVA and GT/PVA nanofibrous mats. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay using L929 fibroblast cells indicated more biocompatibility of the CS-PVA/GT-PVA hybrid nanofibers compared with the CS/PVA and GT/PVA ones. The obtained results showed that the CS-PVA/GT-PVA hybrid nanofibrous scaffold might be useful for tissue engineering applications.