Abstract
The study aims to fabricate and characterize biodegradable membranes, comprising of chitosan/gelatin/ polyvinyl alcohol (PVA) terpolymers to replace the use of synthetic membranes for mammalian cell culture. Blends of chitosan, PVA and gelatin, in varying ratios, were solution cast to fabricate macroporous membranes. The membranes were characterised using Field Emission Scanning Electron Microscopy (FE-SEM) and Fourier Transform Infrared Spectroscopy (FTIR). The influence of different ratios of polymers on membrane properties, such as in vitro degradation, apparent density and water contact angle were investigated. The biocompatibility of the membranes was confirmed using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Further, a mammalian skin keratinocyte (HaCat) cell line was employed to ascertain cellular adhesion and proliferation on the membranes. The developed macroporous membranes supported the adhesion and growth of mammalian cells and were thus found to be suitable and sustainable alternatives to synthetic membranes for in vitro cell culture.
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