Chitosan and Hyaluronic Acid Nanopolyelectrolyte Combined with Tanshinone IIA Targets Signal Transduction Pathway to Prevent Atherosclerosis

Abstract

The purpose of this study was to evaluate the in vitro target impact of manufactured nanocarriers on vascular endothelial cells, as well as the effect of stable concentration and physicochemical properties of chitosan and hyaluronic acid nanoparticles loaded with tanshinone IIA, on the successful adsorption of targeting antibody. Polyelectrolyte composite nanoparticles were prepared by neutralizing chitosan (CS) and hyaluronic acid (HA). Anti-atherosclerosis antibody was quantitatively adsorbed on CS-HA nanoparticles after 4 h in water or PBS. Nanocarriers created in a lab are put through in vivo and in vitro tests on vascular endothelial cells and atherosclerotic plaques. Complexation and physicochemical properties of colloids were affected by external factors including charge mixing ratio and polymer content. The aforementioned method was used to produce non-stoichiometric CS-HA nano-colloids that were stable in water or PBS (pH 7.4) for over a month. Its morphology was analyzed using a scanning electron microscope. Nanoparticles of the CS-HA/CD47 antibody have a positive Zeta potential. Using this nanocarriers in vivo adsorbed to endothelial cells and plaques quite efficiently. Chitosan and hyaluronic acid nanopolyelectrolyte combined with tanshinone IIA nanoparticles were successfully synthesized. Customized nanocarriers may adsorb to endothelial cell lines and atherosclerotic plaques in vitro.