Abstract
Shrimp waste contains 20% - 60% chitin and possible to be source of chitinolytic bacteria. Chitinolytic bacteria are capable of hydrolyzing of chitin progressively to produce N-acetylglucosamine monomer which can be used to overcome the shrimp waste. The objectives of this research were to identify species of bacteria with high activity of chitin degradation in shrimp waste and to analyze their potency as chitin degradation agent. The research consists of screening of chitinolytic bacteria based on chitinolytic index, activity assay of chitinase using colorimetric method, and molecular identification of bacteria based on 16S rDNA sequences. Two of eighteen isolates of chitinolytic bacteria (PBK 2 and SA 1.2 isolates) showed the highest chitinolytic index, which were 2.069 and 2.084, whereas chitinase activity was 0.213 and 0.219 U/ml respectively. Based on 16S rDNA sequences, isolate of PBK 2 was identified as Acinetobacter johnsonii 3-1, whereas SA 1.2 was identified as Bacillus amyloliquefaciens GR53 with 99.78% similarity.
Highlights
Shrimp is one of the important export commodities of Indonesian fishery; many fishery industries provide processed shrimp products to be exported [1]
The objectives of this research were to identify species of bacteria with high activity of chitin degradation in shrimp waste based on 16S rDNA and to analyze their potency as chitin degradation agent
Eighteen isolates with different morphology of colonies were isolated from shrimp waste, 14 isolates from wastewater, and 4 isolates from shrimp-shells waste
Summary
Shrimp is one of the important export commodities of Indonesian fishery; many fishery industries provide processed shrimp products to be exported [1]. How to cite this paper: Setia, I.N. and Suharjono (2015) Chitinolytic Assay and Identification of Bacteria Isolated from Shrimp Waste Based on 16S rDNA Sequences. Chitin (C6H9O4∙NHCOCH)n is linier homopolysaccharide which consists of 2000 - 3000 monomers of β-1,4 linked N-acetyl-D-glucosamine). It is second-most abundant organic compound after cellulose [5]. Chitinolytic bacteria are capable of producing chitinase and hydrolyzing chitin progressively to produce GlNAc (N-acetylglucosamine) monomer through enzymatic reaction [9]. These bacteria are found in soil, marine, lake, or chitinous waste such as industrial shrimp waste [10]. The objectives of this research were to identify species of bacteria with high activity of chitin degradation in shrimp waste based on 16S rDNA and to analyze their potency as chitin degradation agent
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