Chitinases and endoglucanases synthesized in the infected barley leaves in the powdery mildew period sporulation

Abstract

In our work, we represent genetic, molecular and biochemical changes induced in the infected barley leaves induced in the period pathogen sporulation. We studied a presence of acidic β-1,3-endoglucanases and chitinases in an intracellular fluid isolated from primary barley leaves infected with two powdery mildew pathotypes carrying various virulence genes. In the period of pathogen sporulation acidic β-1,3-endoglucanases (Glu) and chitinases (Chi) isozymes were synthesized in two main groups. Non-specific, high molecular group of these enzymes with Mr > 200 kDa did not differ genetic differences between host and pathogen. Specific, low molecular forms reflected both genetic background of host and pathogen as well as biochemical status of the disease. Low molecular acidic Glu had Mr from interval 9–12 kDa. Analogical situation was observed for low molecular chitinases. Their molecular masses were from interval 14.4–15.5 kDa. Intracellular fluid from the primary leaves of the healthy plants did not contained detectable amount of these enzymes. Appearance of low molecular forms of Glu and Chi is discussed.