Abstract
The present investigation aims to isolate and identify chitinase producing mycoflora and the chitinase production by efficient Aspergillus terreus on different shell powder wastes substrates such as prawn, crab and molluscs at an extracellular and intracellular level and its efficacy in assessing the antifungal activity. Shell fish wastes disposed soil samples were collected and screened for chitinolytic fungi (hydrolyzing zone). The chitinase production on different substrates was assessed and the enzyme extracts were screened for antifungal activity by agar well and disc diffusion methods. Among the number of mycoflora isolated only 4 fungal strains Aspergillus terreus, A. fumigatus, A. flavus, and A. clavatus showed maximum hydrolyzing zones. A significantly highest hydrolyzing zone (clearance zone) of 20 mm (out of colony diameter of 65 mm) was obtained by Aspergillus terreus. Among the different substrates (prawn, crab and molluscs shell powders at 1% concentration), highest chitinase activity was recorded by Aspergillus terreus on prawn shell powder waste followed by crab shell powder waste at an intra and extra cellular level. The chitinase produced from prawn shell powder waste as substrates by A. terreus showed maximum zone of inhibition in both agar well (28.0 mm) and disc diffusion (24.0 mm) methods against the pathogenic fungi, A. flavus compared to positive control (Fluconazole). The chitinase produced from A. terreus might have synthesized some antifungal compounds which may be responsible for killing pathogenic fungi.
Highlights
The present investigation aims to isolate and identify chitinase producing mycoflora and the chitinase production by efficient Aspergillus terreus on different shell powder wastes substrates such as prawn, crab and molluscs at an extracellular and intracellular level and
A. terreus showed remarkably prominent clear zone of chitinolytic activity, it was selected as a potential candidate for the enzyme study
Screening of the enzyme extracts for antifungal activity (Table 3) Agar well diffusion method It was observed from the results of the agar well diffusion method that among the enzyme produced from different substrates by Aspergillus terreus, highest zone of inhibition of 28.0 mm was observed against phytopathogenic fungi, Aspergillus flavus from the chitinase produced from the prawn powder waste as a substrate
Summary
The present investigation aims to isolate and identify chitinase producing mycoflora and the chitinase production by efficient Aspergillus terreus on different shell powder wastes substrates such as prawn, crab and molluscs at an extracellular and intracellular level and. The chitinase produced from prawn shell powder waste as substrates by A. terreus showed maximum zone of inhibition in both agar well (28.0 mm) and disc diffusion (24.0 mm) methods against the pathogenic fungi, A.
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