Abstract
Extracellular chitinases play a significant role to stipulate the bioefficacy level of native Pseudomonas isolates against Fusarium udum and Spodoptera litura Fab. Present exertion is to confirm whether extracellular chitinase production is considered as an inducible biocontrol trait in Pseudomonas spp. Pseudomonas aeruginosa p-15 showed maximum chitinase activity in King’s B broth 3.75 U/ml among all seven chitinase positive native isolates from Anand, Gujarat. Enhanced chitinase activity by 28.0, 15.2, and 8.0 % was observed after 84 h when broth was amended with 1.5 % of colloidal chitin, dry powder of tyndallized Fusarium udum and Spodoptera litura (third instar), respectively. The components of King’s B medium were modified and used for chitinase expression as optimized using response surface methodology (RSM) for the responses such as colony count, chitinase activity, and biocontrol activity. These efforts suggest chitinase as an inducible trait in Pseudomonas aeruginosa p-15.
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