Chitinase Activity ofSerratia marcescensPT-6 Cultured in Bioreactor with Various Aeration Rate

Niswah Umhudloh Dzakiyya,Sri Pudjiraharti,Indun Dewi Puspita,A Isnansetyo,R Ida Adharini,Mgs M Prima Putra,N Huda,D Wahyu Kartika Sari,R.C Bhujel,A Dwi Jayanti,I Dewi Puspita

doi:10.1051/e3sconf/202014703010

Abstract

Chitin is a biopolymer composed of N-acetylglucosamine (NAG) monomers. Bacterial chitinase helps in bioconversion process of chitin from shrimp shell waste into NAG. This study aimed to determine the effect of aeration on chitinase activity, NAG concentration in medium, bacterial growth, and pH medium; to determine the optimum aeration on chitinase production ofSerratia marcescensPT-6. Chitinase was produced using a 1.5 L bioreactor at pH 7, 30°C, 200 rpm agitation speed, and variation of aeration rates (0.4, 0.6 and 0.8 vvm). Parameters examined included pH, bacterial growth (log CFU/ml), chitinase activity (U/ml), and NAG concentration in medium (µg/ml). Bacterial growth was measured by enumeration of bacterial colony in medium, while chitinase activity and NAG concentration were analyzed by colorimetric assay. Parameters was measured every 8 hours during 60 hours of incubation. Aeration rate showed a significant effect (P≤0.05) on chitinase activity and NAG concentration, but not significant (P≥0.05) on bacterial growth and pH medium. Optimum aeration rate on producing chitinase was 0.6 vvm with the highest chitinase activity of 0.0125 U/ml at 40 hours incubation. This study implies that optimization of bioreactor condition improved the production of chitinase byS. marcescensPT-6.

Highlights

Chitin is a biopolymer composed of N-acetylglucosamine (NAG) monomers and linked by ß-1.4 glycosidic bonds [1]
Chitinase is produced by bacteria, fungi, plants, and animals [7]
This study aimed to determine the effect of aeration on chitinase activity, NAG concentration in medium, bacterial growth, and pH medium

Summary

Introduction

Chitin is a biopolymer composed of N-acetylglucosamine (NAG) monomers and linked by ß-1.4 glycosidic bonds [1]. Shrimp shells are wastes that cause environmental pollution and have low economic value [3]. Bioconvertion process of shrimp shell waste into chitin or hydrolysis products is needed to provide value added and reduce environmental pollution [4]. An enzyme that capable in degrading ß-1.4-glycosidic bond of chitin, is used in the biological process of chitin hydrolysis. S. marcescens produces more than 1 type of chitinase enzyme [9]. S. marcescens chitinase showed specific characteristics and synergistic activities in degrading chitin [10]. S. marcescens has been known as a good producer of chitinase with high chitinase activity, i.e. 35.2 U/ml [11], 61.01 U/ml [12], and 77.3 U/ml [13]

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Results