Abstract
The rotation angle/absorbance ratios C += α +/ A + and C −= α −/ A −, determined via detection by a polarimeter and a photometer, were checked for the first time with reference to their use for on-line analysis during preparative separations. For this purpose, (+)-, (−)- and (±)-carvones were investigated by liquid chromatography (LC) on microcrystalline tribenzoylcellulose. It turned out that the ratios C + and C − depend only slightly upon concentration ( Table 1). Overlapped peaks of enantiomers were successfully submitted to computer deconvolution (e.g. Fig. 2, bottom). A procedure for on-line analysis during preparative LC is proposed.
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