Abstract
This paper presents a new ecotoxicological test to investigate the response of a Brazilian endemic insect Chironomus sancticaroli through its life cycle and its future generations. This test can evaluate differences between the endpoints analyzed in diverse generations, describing the long-term impact of a substance or matrix effects along the time of exposure. Despite earlier papers already present the generation test with Chironomus riparius, there are still no studies with long-term test applied to C. sancticaroli. In this sense, this study evaluated different conditions for the development of a methodology that prolonged the duration of the test and allowed the best sampling of the organism on environmental toxicity tests. The distinct conditions tested were: volumes of test solution, frequency of feed, number of larvae, type of vessel and test solution replacement. The best condition for the C. sancticaroli generation test includes the exposition of 20 larvae to 340 or 500 mL of test solution, 60 g formulated sediment, feeding with Tetramin® each 10 days and, from a spawn, a new test will be prepared with the same characteristics of the previous one. This new methodology can reveal toxic effects along the exposure time and brings advances on toxicology area.•Prolonged testing makes it possible to analyze the long-term effects•The methodology "C. sancticaroli generation test" allows to evaluate the responses of organisms in different life cycles•The methodology allows to analyze the effects of substances in liquid medium and environmental quality through exposure to sediment
Highlights
In order to evaluate this gap, the present study proposes a methodology for the creation and accomplishment of long-term toxicity tests using C. sancticaroli (C. xanthus or C. domizii) in the laboratory, with the objective of covering more than one generation of the species and contributing to refine lethal and sublethal effects of diverse substances to the aquatic biota
The organisms cultivated in laboratory were used to evaluate the best condition to proceed the C. sancticaroli generation test
This long-term ecotoxicological tests were carried out involving the entire life cycle of the C. sancticaroli species
Summary
Chironomus sancticaroli generation test: A new methodology with a Brazilian endemic insect. Mara R. de Lima e Silva, Juliano J.
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