Abstract
A matrix that enabled chirality and structure-selective detection in matrix assisted laser desorption ionization mass spectrometry (MALDI MS) has been developed. Molds of L- or D- alanine were made on a thermoreversible polymer (polyvinyl methyl ether) with 2,4,6-trihydroxyacetophenone, and this was used as a matrix. Separate detection of one optical isomer of alanine was realized in MALDI MS. This technique was also applied to the detection of trisaccharides having the same molecular weight but different structures. Separate detection of raffinose and maltotriose in MALDI MS were presented.
Highlights
The importance of dealing with enantiomers is increasing in many research fields, including medicine, agriculture, and food, because enantiomers exhibit different biological activities
Mass spectrometry used in combination with various separation techniques, including gas chromatography-mass spectrometry (GC-MS)[1] and liquid chromatography-mass spectrometry (LC-MS)[2,3], has been employed
As L-Ala is water-soluble, L-Ala exists at the outer surface of Polyvinyl methyl ether (PVME) facing the solution
Summary
The importance of dealing with enantiomers is increasing in many research fields, including medicine, agriculture, and food, because enantiomers exhibit different biological activities. We present the separate detection of alanine by using a newly developed chirality-sensitive matrix.
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