CHIRAL STATIONARY PHASES BASED ON CELLULOSE AND AMYLOSE TRIS-3,5-DIMETHYLPHENYLCARBAMATE DERIVATIVES FOR THE RESOLUTION OF SELECTED CHIRAL DRUGS AND METABOLITES

Abstract

Chiral stationary phases based on tris-3,5-dimethyl-phenylcarbamate derivatives of cellulose and amylose were used for the resolution of some chiral drugs and their metabolites. The Chiralcel OD-H and Chiralpak AD columns were employed under normal phase conditions using hexane/ethanol or hexane/2-propanol as the mobile phases. The influence of trace amounts of diethylamine in the mobile phase was also evaluated. The Chiralcel OD-R column was employed under reversed phase condition using mainly perchlorate solution supplemented with acetonitrile. The results showed that the resolution obtained under normal phase conditions in general is better than that obtained using the same kind of stationary phase under reversed phase conditions. The Chiralpak AD provided the best resolution for all drugs studied.