Chiral Separation of Amlodipine and its Enantiomer on a Molecularly Imprinted Polymer-Based Stationary Phase

Abstract

A new chiral stationary phase for amlodipine (3-ethyl 5-methyl-2 -[(-2-(aminoethoxymethyl)-4-(2-chlorophenyl)-1,4-dihydro-6-methyl-3,5-pyridinedicarboxylate) based on a molecularly imprinted polymer has been prepared by radiation polymerization method and used for chromatographic separation of the chiral enantiomers. The effects on the separation of mobile phase and flow rate were investigated, and the optimum conditions for HPLC were shown to be: mobile phase, chloroform; flow rate, 0.3 ml min-1; at room temperature. The results has shown that the amlodipine-imprinted polymer was capable of recognizing the enantiomeric difference between the template and its R-enantiomer, whereas the non-imprinted and thermal polymerization polymer have no chiral recognition ability for the enantiomers.