Chiral separation of amino acids by capillary electrophoresis with octyl-beta-thioglucopyranoside as chiral selector.

Abstract

1-S-Octyl-beta-D-thioglucopyranoside (OTG) was evaluated as a chiral selector for the separation of dansyl-amino acids by capillary electrophoresis. Enantiomeric separations of the amino acids can be accomplished by judiciously adjusting the pH of the solution and the concentration of OTG. Better separation can be achieved, however, when OTG is used, not as a sole chiral selector but rather together with sodium dodecyl sulfate (SDS) and cyclodextrin (CD). Interestingly, not only can this OTG-SDS-CD system provide better separation than OTG alone but also it can separate compounds which cannot be separated when only CD or CD and SDS are used. All amino acids were baseline separated with this OTG-SDS-CD system at optimal conditions.